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Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Product features
genesig® kits are sold for research use only and are not licensed for diagnostic procedures.
Advanced kit contents:
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control - Read through VIC channel* (150 tests)
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
Standard kit contents:
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
RNAse/DNAse free water
Total cellular DNA samples were amplified using the Genesig Advanced Kit for quantification of hepatitis B virus genomes (PrimerDesign™Ltd., Southampton, UK) and Applied Biosystems® 7500 Fast Real-time PCR System.
Kumamoto H, Higashi-Kuwata N, Hayashi S, Das D, Bulut H, Tokuda R, Imoto S, Onitsuka K, Honda Y, Odanaka Y, Shimbara-Matsubayashi S, Haraguchi K, Tanaka Y, Mitsuya H. Synthesis of novel entecavir analogues having 4'-cyano-6''-fluoromethylenecyclopentene skeletons as an aglycone moiety as highly potent and long-acting anti-hepatitis B virus agent. RSC Adv. 2023 May 31;13(23):15999-16011. doi: 10.1039/d3ra01750h. PMID: 37265996; PMCID: PMC10230191.
The plasma viral load was determined by Real-Time PCR using the 7500 Fast Real time PCR device with the Genesig HBV Real Time Quantitative Kit Primer design (Southampton, United Kingdom) by amplification of the Core Protein Region.
Bazie MM, Djigma FW, Sanou M, Sorgho PA, Ouattara AK, Obiri-Yeboah D, Kapieko N, Sombie HK, Bado P, Yelemkoure ET, Kiendrebeogo IT, Nagalo MB, Yonli AT, Simpore J. Killer cell immunoglobulin-like receptor alleles influence susceptibility to occult hepatitis B infection in West African population. J Public Health Afr. 2023 Oct 1;14(9):2586. doi: 10.4081/jphia.2023.2586. PMID: 37908389; PMCID: PMC10615156.
Viral loads were quantified for each sample using the HBV Genesig® standard kit (PrimerdesignTM Ltd., Eastleigh, UK), which targets a region in the core gene.
Phan NMH, Faddy HM, Flower RL, Dimech WJ, Spann KM, Roulis EV. Low Genetic Diversity of Hepatitis B Virus Surface Gene amongst Australian Blood Donors. Viruses. 2021 Jun 30;13(7):1275. doi: 10.3390/v13071275. PMID: 34208852; PMCID: PMC8310342.
PCR was conducted using a genesig standard kit for HBV core protein region (Primerdesign Ltd., Southampton, UK).
Higashi-Kuwata N, Hayashi S, Das D, Kohgo S, Murakami S, Hattori SI, Imoto S, Venzon DJ, Singh K, Sarafianos SG, Tanaka Y, Mitsuya H. CMCdG, a Novel Nucleoside Analog with Favorable Safety Features, Exerts Potent Activity against Wild-Type and Entecavir-Resistant Hepatitis B Virus. Antimicrob Agents Chemother. 2019 Mar 27;63(4):e02143-18. doi: 10.1128/AAC.02143-18. PMID: 30670420; PMCID: PMC6437475.
Plasma viral load was determined using the 7500Fast Real Time PCR system (Applied Biosystems, USA) using the Genesig HBV Real Time Quantitative Kit Primer design kit (Southampton, United Kingdom).
Sorgho PA, Martinson JJ, Djigma FW, Yonli AT, Nagalo BM, Compaore TR, Obiri-Yeboah D, Diarra B, Sombie HK, Zongo AW, Ouattara AK, Soubeiga STR, Traore L, Roberts LR, Simpore J. Insights into the Interplay between KIR Gene Frequencies and Chronic HBV Infection in Burkina Faso. Mediterr J Hematol Infect Dis. 2018 Nov 1;10(1):e2018060. doi: 10.4084/MJHID.2018.060. PMID: 30416692; PMCID: PMC6223576.
Total cellular DNA samples were amplified using the genesig Advanced Kit for quantification of Hepatitis B Virus genomes (PrimerDesign™Ltd., Southampton, UK) and Applied Biosystems®7500 Fast Real-time PCR System (Life Technologies Japan Ltd., Tokyo Japan).
Onitsuka K, Tokuda R, Kuwata-Higashi N, Kumamoto H, Aoki M, Amano M, Kohgo S, Das D, Haraguchi K, Mitsuya H, Imoto S. Synthesis and evaluation of the anti-hepatitis B virus activity of 4'-Azido-thymidine analogs and 4'-Azido-2'-deoxy-5-methylcytidine analogs: structural insights for the development of a novel anti-HBV agent. Nucleosides Nucleotides Nucleic Acids. 2020;39(4):518-529. doi: 10.1080/15257770.2019.1664749. Epub 2019 Sep 12. PMID: 31514570; PMCID: PMC8191380.
HBV viral load was determined using Genesig HBV Real Time Quantitative kit (Primerdesign, Southampton, United Kingdom).
Compaore TR, Diarra B, Assih M, Obiri-Yeboah D, Soubeiga ST, Ouattara AK, Tchelougou D, Bisseye C, Bakouan DR, Compaore IP, Dembele A, Djigma WF, Simpore J. HBV/HIV co-infection and APOBEC3G polymorphisms in a population from Burkina Faso. BMC Infect Dis. 2016 Jul 22;16:336. doi: 10.1186/s12879-016-1672-2. PMID: 27449138; PMCID: PMC4957463.
HBV/HIV co-infection and APOBEC3G polymorphisms in a population from Burkina Faso - PMC (nih.gov)
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