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Primer Design

New expanded range of Master Mixes

Unlocking Precision in Every Drop: Elevate Your Diagnostic Potential with Our Superior qPCR Master Mixes!

Our range of sensitive and accurate master mixes offer unique enzyme/buffer combinations that allow you to get the best from your testing. Whether you are looking for fast results, optimal multiplexing capability, or a reliable cost-effective testing process, our master mixes are developed to suit you and your testing requirements.

Precision Plus® Precision Plus® Onestep Precision Fast® oasig® Standard oasig® Onestep oasig® PLUS NEW
Primer Design Primer Design Primer Design Primer Design Primer Design Primer Design
Application Gene expression analysis (cDNA)Speciation testing. Pathogen detection (bacteria, fungi, DNA viruses, parasites) Gene expression analysis (RNA) RNA pathogen detection including RNA viruses. Rapid screening High throughput pathogen detection (bacteria, fungi, DNA viruses) and genomic or cDNA target detection Pathogen detection (bacteria, fungi, DNA viruses, parasites), genotyping and genomic target detection Multiplexing. Gene expression studies from cDNA. Gene expression studies directly from RNA and pathogen detection (bacteria, fungi, RNA/DNA viruses, parasites). Gene expression analysis. Pathogen detection Multiplexing of RNA or DNA targets
+SYBR Green Yes Yes Yes n/a n/a n/a
+ROX* Yes Yes Yes Yes Yes Yes
Concentration 2x 2x 2x 2x 2x 2x
Target DNA RNA DNA DNA RNA RNA
Fast Cycling n/a RNA DNA DNA RNA RNA
Multiplex n/a n/a n/a +++ n/a +++

*Rox passive reference dye not suitable for multiplex assays above 2 targets

Mastermix Selection Tool

New expanded range of oasig Master Mixes

Primer Design
Primer Design
Primer Design

oasigPLUS OneStep Lyophilised Master Mix

oasigPLUS introduces a unique technology that eliminates reverse transcriptase activity at room temperature resulting in improved multiplexing results and superior performance.

Multiplex RNA assays are of an increased risk of non- specific artefact formation (eg primer dimers) due to increased number of primers.

This is due to the Reverse transcriptase (RTase), the enzyme required to drive the PCR of RNA targets, being active at room temperature during reaction setup when primers are bound in a non-specific fashion.

oasigPLUS OneStep Master Mix utilises tightly controlled temperature dependent reverse transcriptase enzyme technology. Unwanted primer interactions during room temperature set-up are therefore removed resulting in improved multiplexing performance.

Features

  • For use with RNA and DNA assays
  • Increased sensitivity at low copy
  • Optimal multiplexing performance
  • Improved endpoint/ reduction of artefact formation
Primer Design
Catalogue No. Product Description Kit Size
Z-PFAST-1ml; 2ml; 5ml; 10ml; 20ml Precision FAST qPCR Master Mix 1ml; 2ml; 5ml; 10ml; 20ml View more
Z-PPLUS-1ml; 2ml; 5ml; 10ml; 20ml Precision PLUS qPCR Master Mix 1ml; 2ml; 5ml; 10ml; 20ml View more
Z-OSPLUS-1ml; 2ml; 5ml; 10ml; 20ml Precision Plus OneStep RT-qPCR Master Mix 1ml; 2ml; 5ml; 10ml; 20ml View more
Z-oasig-standard-150 oasig Lyophilised 2X qPCR Master Mix 150rxn View more
Z-oasig-onestep-150 oasig Lyophilised OneStep 2XRT-qPCR Master Mix 150 rxn View more
R01070, R01071 oasigPLUS OneStep Lyophilised 2X RT-qPCR Master Mix 50, 150 rxn View more
Browse all Master Mix Download our brochure

Precision Master Mix selection table

PFAST / PPLUS / XX / XX / XML
Primer Design
Mastermix type
Primer Design
Choose between: PFAST, PPLUS and OSPLUS
PFAST / PPLUS / XX / XX / XML
Primer Design
Passive reference dye
Primer Design
Choose between: no prefix, LR, R, MX, CL or iC (depending on instrument)

No Prefix

No ROX for: q16, q32, BioRad CFX96, Opticon and Chromo4 (all models), RotorGene, Roche LightCycler 96 and 480, Eppendorf Mastercycler, Fluidigm BioMark, Cepheid SmartCycler, Illumina Eco, PCRMax Eco, Thermo PikoReal, PrimePro 48, Analytik Jena qTower Series

LR

Low ROX for: ABI 7500 (Fast), ABI ViiA7, Life Technologies QuantStudio (QS 3, 5, 6, 7, 12)

R

Standard ROX for: ABI 7000, ABI 7300, ABI 7700, ABI 7900, StepOne, StepOnePLUS, GeneAMP 5700

MX

Medium ROX for: Stratagene MX (all models)

CL

Standard ROX and OsrHSA for: Capillary Lightcycler (Roche LightCycler 1.0-2.0)

iC

Fluorescein for: BioRad iCycler (all models)

PFAST / PPLUS / XX / XX / XML
Primer Design
SYBR needed?
Primer Design
Choose between: No prefix (for probe- based assays) SY (for SYBR assays)
PFAST / PPLUS / XX / XX / XML
Primer Design
Volume
Primer Design
Choose between: 1ML, 2ML, 5ML, 10ML and 20ML

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