Our range of sensitive and accurate master mixes offer unique enzyme/buffer combinations that allow you to get the best from your testing. Whether you are looking for fast results, optimal multiplexing capability, or a reliable cost-effective testing process, our master mixes are developed to suit you and your testing requirements.
| Precision Plus® | Precision Plus® Onestep | Precision Fast® | oasig® Standard | oasig® Onestep | oasig® PLUS NEW | |
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| Application | Gene expression analysis (cDNA)Speciation testing. Pathogen detection (bacteria, fungi, DNA viruses, parasites) | Gene expression analysis (RNA) RNA pathogen detection including RNA viruses. | Rapid screening High throughput pathogen detection (bacteria, fungi, DNA viruses) and genomic or cDNA target detection | Pathogen detection (bacteria, fungi, DNA viruses, parasites), genotyping and genomic target detection Multiplexing. Gene expression studies from cDNA. | Gene expression studies directly from RNA and pathogen detection (bacteria, fungi, RNA/DNA viruses, parasites). | Gene expression analysis. Pathogen detection Multiplexing of RNA or DNA targets |
| +SYBR Green | Yes | Yes | Yes | n/a | n/a | n/a |
| +ROX* | Yes | Yes | Yes | Yes | Yes | Yes |
| Concentration | 2x | 2x | 2x | 2x | 2x | 2x |
| Target | DNA | RNA | DNA | DNA | RNA | RNA |
| Fast Cycling | n/a | RNA | DNA | DNA | RNA | RNA |
| Multiplex | n/a | n/a | n/a | +++ | n/a | +++ |
*Rox passive reference dye not suitable for multiplex assays above 2 targets
Mastermix Selection Tool
oasigPLUS introduces a unique technology that eliminates reverse transcriptase activity at room temperature resulting in improved multiplexing results and superior performance.
Multiplex RNA assays are of an increased risk of non- specific artefact formation (eg primer dimers) due to increased number of primers.
This is due to the Reverse transcriptase (RTase), the enzyme required to drive the PCR of RNA targets, being active at room temperature during reaction setup when primers are bound in a non-specific fashion.
oasigPLUS OneStep Master Mix utilises tightly controlled temperature dependent reverse transcriptase enzyme technology. Unwanted primer interactions during room temperature set-up are therefore removed resulting in improved multiplexing performance.
| Catalogue No. | Product Description | Kit Size | |
|---|---|---|---|
| Z-PFAST-1ml; 2ml; 5ml; 10ml; 20ml | Precision FAST qPCR Master Mix | 1ml; 2ml; 5ml; 10ml; 20ml | View more |
| Z-PPLUS-1ml; 2ml; 5ml; 10ml; 20ml | Precision PLUS qPCR Master Mix | 1ml; 2ml; 5ml; 10ml; 20ml | View more |
| Z-OSPLUS-1ml; 2ml; 5ml; 10ml; 20ml | Precision Plus OneStep RT-qPCR Master Mix | 1ml; 2ml; 5ml; 10ml; 20ml | View more |
| Z-oasig-standard-150 | oasig Lyophilised 2X qPCR Master Mix | 150rxn | View more |
| Z-oasig-onestep-150 | oasig Lyophilised OneStep 2XRT-qPCR Master Mix | 150 rxn | View more |
| R01070, R01071 | oasigPLUS OneStep Lyophilised 2X RT-qPCR Master Mix | 50, 150 rxn | View more |
No Prefix
No ROX for: q16, q32, MyGo Mini, MyGo Pro (ESR), BioRad CFX96, Opticon and Chromo4 (all models), RotorGene, Roche LightCycler 96 and 480, Eppendorf Mastercycler, Fluidigm BioMark, Cepheid SmartCycler, Illumina Eco, PCRMax Eco, Thermo PikoReal, PrimePro 48, Analytik Jena qTower Series
LR
Low ROX for: ABI 7500 (Fast), ABI ViiA7, Life Technologies QuantStudio (QS 3, 5, 6, 7, 12)
R
Standard ROX for: ABI 7000, ABI 7300, ABI 7700, ABI 7900, StepOne, StepOnePLUS, GeneAMP 5700
MX
Medium ROX for: Stratagene MX (all models)
CL
Standard ROX and OsrHSA for: Capillary Lightcycler (Roche LightCycler 1.0-2.0)
iC
Fluorescein for: BioRad iCycler (all models)