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Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Product features
genesig® kits are sold for research use only and are not licensed for diagnostic procedures.
Advanced kit contents:
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control - Read through VIC channel* (150 tests)
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
Standard kit contents:
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
RNAse/DNAse free water
HAV (target/5′ NCR), HCV (5′UTR), HEV (ORF2), HIV-1 (target/POL), HIV-2 (target/POL), HTLVI (target/POL), HTLVII (target/POL), and WNV (5′UTR) positive specimens were quantified using the Primer Design Genesig kit (Primerdesign Ltd, United Kingdom) according to the manufacturer’s protocol (OneStep RT-qPCR protocol). Each kit contained a positive control template for the PCR set up and for copy number determination (generated serial dilutions for the standard curve). The RT-qPCR assays were performed on a ViiA7 Applied Biosystems real-time PCR system.
De Giorgi V, Zhou H, Alter HJ, Allison RD. A microarray-based pathogen chip for simultaneous molecular detection of transfusion-transmitted infectious agents. J Transl Med. 2019 May 14;17(1):156. doi: 10.1186/s12967-019-1905-4. PMID: 31088488; PMCID: PMC6518760.
The genesig real-time PCR detection kit from Primerdesign™ Ltd. supplied by oasig lyophilized One-Step RT-qPCR MasterMix was used for HEV detection (based on the detection of the ORF2 capsid protein-encoding gene).
Bazir H, Hassou N, El Mellouli F, Zekhnini H, Najib S, Ennaji MM. Hepatitis A and E Viruses in Mussels from Cherrat Estuary in Morocco: Detection by Real-Time Reverse Transcription PCR Analysis. Adv Virol. 2022 Nov 28;2022:8066356. doi: 10.1155/2022/8066356. PMID: 36479562; PMCID: PMC9722280.
The selected plasma batch was tested for the presence of PCV-2 genome and antibodies, as indicated previously, and was also analyzed for the presence of other pathogens by commercial qPCR or qRT-PCR techniques, including PRRSV (LSI VetMAXTM PRRSV EU/NA Real-Time PCR Kit. Thermo Fisher Scientific, Massachusetts, USA), TGEV (EXOone TGEV, EXOPOL, Zaragoza, Spain), SIV (EXOone Influenza A, EXOPOL, Zaragoza, Spain), PPV (EXOone Parvovirus, EXOPOL, Zaragoza, Spain), PEDV (EXOone PEDV, EXOPOL, Zaragoza, Spain), RVA (EXOone Rotavirus A, EXOPOL, Zaragoza, Spain), HEV (Path-HEV advance from Genesig-Primerdesign, Cambridge, UK), and S. enterica (EXOone Salmonella enterica, EXOPOL, Zaragoza, Spain).
Blázquez E, Rodríguez C, Ródenas J, Navarro N, Rosell R, Pina-Pedrero S, Campbell JM, Sibila M, Segalés J, Pujols J, Polo J. UV-C irradiation is able to inactivate pathogens found in commercially collected porcine plasma as demonstrated by swine bioassay. Vet Microbiol. 2019 Dec;239:108450. doi: 10.1016/j.vetmic.2019.108450. Epub 2019 Oct 12. PMID: 31753544; PMCID: PMC7130610.
The developed system of HEV RNA detection both in real-time RT-PCR and in a nested PCR variant has confirmed its sensitivity to the synthetic reference templates and positive control samples in commercial test system (Genesig, Great Britain).
Voronina OL, Kunda MS, Gudov VP, Aksenova EI, Shilova VS, Yarosh LV, Elgort DA, Lunin VG, Semenenko TA. [Search for RNA of the hepatitis E virus autochthonous for Russia in the most likely infection sources]. Mol Gen Mikrobiol Virusol. 2014;(3):29-34. Russian. PMID: 25335410.
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