Leptospirosis

Kit

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Product Description
Ordering
Resources

Information

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Product Features

Product features

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings

genesig® kits are sold for research use only and are not licensed for diagnostic procedures.

Advanced kit contents:

Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control - Read through VIC channel* (150 tests)
Endogenous control (150 tests)
RNAse/DNAse free water

*alternative fluorophores available on request

Standard kit contents:

Ordering

We work hard to keep shipping costs to a minimum. Kits are normally sent by courier to ensure rapid delivery. Cost varies according to your location. If you are using the online shopping facility this will be calculated for you automatically, otherwise please contact us for detailed pricing.

We sell our products across the globe. You will either have the option to purchase through us directly or through a trusted distributor, depending on your location.

Please enquire for the alternative Standard, Advanced, or Easy Kit formats if not listed. Kit formats not listed will be made to order and typically have a dispatch time of 4-6 weeks.

Resources

Advanced Kit Handbook Easy Kit Handbook Standard Kit Handbook

Fresh kidney samples (n = 64) were tested for Leptospira using polymerase chain reaction (PCR) at VDL. DNA extraction and amplification were performed using the Qiagen QIAamp DSP Virus Kit (Qiagen, Hilden, Germany) and the outer membrane protein lipl32 genesig Advanced Kit following the manufacturer's protocol (Primerdesign, Chandler's Ford, United Kingdom).

Flay KJ, Yang DA, Wilson MT, Lee SH, Bhardwaj V, Hill FI, Pfeiffer DU. Absence of serological or molecular evidence of Leptospira infection in farmed swine in the Hong Kong Special Administrative Region. One Health. 2021 Aug 30;13:100321. doi: 10.1016/j.onehlt.2021.100321. PMID: 34504940; PMCID: PMC8411228.

Absence of serological or molecular evidence of Leptospira infection in farmed swine in the Hong Kong Special Administrative Region - PMC (nih.gov)

The Genesig® standard kit for quantification of leptospirosis genomes (Primerdesign Ltd, UK) which targets the outer membrane lipl32 was used to for detection of Leptospira by qPCR.

Pedersen K, Anderson TD, Bevins SN, Pabilonia KL, Whitley PN, Virchow DR, Gidlewski T. Evidence of leptospirosis in the kidneys and serum of feral swine (Sus scrofa) in the United States. Epidemiol Infect. 2017 Jan;145(1):87-94. doi: 10.1017/S0950268816002247. Epub 2016 Oct 4. PMID: 27697080; PMCID: PMC9507390.

Evidence of leptospirosis in the kidneys and serum of feral swine (Sus scrofa) in the United States - PMC (nih.gov)

Leptospirosis

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