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Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Product features
genesig® kits are sold for research use only and are not licensed for diagnostic procedures.
Advanced kit contents:
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control - Read through VIC channel* (150 tests)
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
Standard kit contents:
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
RNAse/DNAse free water
HAV (target/5′ NCR), HCV (5′UTR), HEV (ORF2), HIV-1 (target/POL), HIV-2 (target/POL), HTLVI (target/POL), HTLVII (target/POL), and WNV (5′UTR) positive specimens were quantified using the Primer Design Genesig kit (Primerdesign Ltd, United Kingdom) according to the manufacturer’s protocol (OneStep RT-qPCR protocol). Each kit contained a positive control template for the PCR set up and for copy number determination (generated serial dilutions for the standard curve). The RT-qPCR assays were performed on a ViiA7 Applied Biosystems real-time PCR system.
De Giorgi V, Zhou H, Alter HJ, Allison RD. A microarray-based pathogen chip for simultaneous molecular detection of transfusion-transmitted infectious agents. J Transl Med. 2019 May 14;17(1):156. doi: 10.1186/s12967-019-1905-4. PMID: 31088488; PMCID: PMC6518760.
HAV RNA sequence detection by a Genesig HAV Real-Time PCR kit. The sequence selected is proprietary but HAV specific.
Franklin N, Camphor H, Wright R, Stafford R, Glasgow K, Sheppeard V. Outbreak of hepatitis A genotype IB in Australia associated with imported frozen pomegranate arils. Epidemiol Infect. 2019 Jan;147:e74. doi: 10.1017/S0950268818003515. PMID: 30869018; PMCID: PMC6518746.
HDV viral load was determined with Primerdesign HDV genesig assay (Primerdesign Ltd, United Kingdom) which is characterized by high priming efficiencies of >95% and can detect less than 100 copies of target template and was validated with an external control program (QCMD HDV14, QCMD, Glasgow, Scotland).
Shirazi R, Ram D, Rakovsky A, Bucris E, Gozlan Y, Lustig Y, Shaked-Mishan P, Picard O, Shemer-Avni Y, Ben-Zvi H, Halutz O, Lurie Y, Veizman E, Carlebach M, Braun M, Naftaly MC, Shlomai A, Safadi R, Mendelson E, Sklan EH, Ben-Ari Z, Mor O. Characterization of hepatitis B and delta coinfection in Israel. BMC Infect Dis. 2018 Feb 27;18(1):97. doi: 10.1186/s12879-018-3008-x. PMID: 29486716; PMCID: PMC6389180.
Characterization of hepatitis B and delta coinfection in Israel - PMC (nih.gov)
Qualitative HDV RNA PCR was done by reverse transcription-polymerase chain reaction (RT-PCR) using Primer Design™ genesig Kit for Hepatitis D Virus (Primerdesign, UK).
Sayad B, Naderi Y, Alavian SM, Najafi F, Janbakhsh A, Mansouri F, Vaziri S, Afsharian M, Norooznezhad F. Hepatitis D virus infection in Kermanshah, west of Iran: seroprevalence and viremic infections. Gastroenterol Hepatol Bed Bench. 2018 Spring;11(2):145-152. PMID: 29910856; PMCID: PMC5990919.
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