Dengue Subtyping Multiplex Kit

https://www.bioresearchcommunications.com/index.php/brc/article/view/72/78

Co-circulation of Three Dengue Virus Serotypes in 2017 in Dhaka city: First report from BangladeshMizanur Rahman*1, Rummana Rahim1, Abu Hasan1, Abu Sobhan Murad1, Malay Biswas1 1Apollo Hospitals Dhaka Plot-81, Block-E, Bashundhara R/A, Dhaka-1229 Bangladesh

Viral RNA was extracted from patient serum samples, DENV-positive samples were then analyzed using genesig dengue subtyping multiplex kit

Poltep K, Phadungsombat J, Nakayama EE, Kosoltanapiwat N, Hanboonkunupakarn B, Wiriyarat W, Shioda T, Leaungwutiwong P. Genetic Diversity of Dengue Virus in Clinical Specimens from Bangkok, Thailand, during 2018-2020: Co-Circulation of All Four Serotypes with Multiple Genotypes and/or Clades. Trop Med Infect Dis. 2021 Sep 4;6(3):162. doi: 10.3390/tropicalmed6030162. PMID: 34564546; PMCID: PMC8482112. 

Genetic Diversity of Dengue Virus in Clinical Specimens from Bangkok, Thailand, during 2018–2020: Co-Circulation of All Four Serotypes with Multiple Genotypes and/or Clades - PMC (nih.gov)

Serum samples were collected from DENV-positive patients and DENV-positive RNA samples were then analyzed according to the manufacturer’s instructions using a commercial dengue subtyping multiplex kit (Genesig, Chandler’s Ford, UK) for DENV serotype determination.

Poltep K, Nakayama EE, Sasaki T, Kurosu T, Takashima Y, Phadungsombat J, Kosoltanapiwat N, Hanboonkunupakarn B, Suwanpakdee S, Imad HA, Srimark N, Kitamura C, Yamanaka A, Okubo A, Shioda T, Leaungwutiwong P. Development of a Dengue Virus Serotype-Specific Non-Structural Protein 1 Capture Immunochromatography Method. Sensors (Basel). 2021 Nov 24;21(23):7809. doi: 10.3390/s21237809. PMID: 34883813; PMCID: PMC8659457.

Development of a Dengue Virus Serotype-Specific Non-Structural Protein 1 Capture Immunochromatography Method - PMC (nih.gov)

Viral RNA was extracted from 0.2 ml of serum, For DENV serotype identification, we used the commercial Genesig one-step reverse transcriptase real-time PCR kit from Primerdesign with the Rotor Gene Q thermocycler according to the kit manufacturer's instructions.  

Suzuki K, Phadungsombat J, Nakayama EE, Saito A, Egawa A, Sato T, Rahim R, Hasan A, Lin MY, Takasaki T, Rahman M, Shioda T. Genotype replacement of dengue virus type 3 and clade replacement of dengue virus type 2 genotype Cosmopolitan in Dhaka, Bangladesh in 2017. Infect Genet Evol. 2019 Nov;75:103977. doi: 10.1016/j.meegid.2019.103977. Epub 2019 Jul 24. PMID: 31351235.

Genotype replacement of dengue virus type 3 and clade replacement of dengue virus type 2 genotype Cosmopolitan in Dhaka, Bangladesh in 2017 - ScienceDirect

Viral RNA was extracted from serum, Dengue serotype was determined using a Genesig dengue subtyping multiplex kit using a CFX96TM real-time PCR cycler

Imad HA, Phadungsombat J, Nakayama EE, Chatapat L, Pisutsan P, Matsee W, Piyaphanee W, Nguitragool W, Shioda T. A Cluster of Dengue Cases in Travelers: A Clinical Series from Thailand. Trop Med Infect Dis. 2021 Aug 14;6(3):152. doi: 10.3390/tropicalmed6030152. PMID: 34449752; PMCID: PMC8396219.

A Cluster of Dengue Cases in Travelers: A Clinical Series from Thailand - PMC (nih.gov)