oasigPLUS OneStep Lyophilised Master Mix 150

Primerdesign oasigPLUS Onestep Lyophilised 2x RT qPCR Master Mix is an optimised complete Master Mix for use in one step real time PCR. Use of a one step qPCR reaction, including a Reverse Transcription enzyme, reduces handling errors, and greatly shortens the time taken to obtain results. oasigPLUS OneStep Master Mix utilises a tightly controlled temperature dependent Reverse Transcriptase enzyme. This improves multiplexing performance with highter consistency by minimising uwanted non- specific priming and extension during room temperature set-up. The Master Mix contains a superior Reverse Transcriptase (as described above) and a hot start Taq DNA polymerase as well as buffer, dNTPs, MgCl2 and stabilisers at concentrations optimised for the enzymes and qPCR reaction. Once resuspended only the template RNA and primer/probe mix are required to complete the experimental set up for a perfect multiplex reaction.