Equine Flu H3N8 & H7N7
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Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
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Nasal swab samples were collected from horses and tested for equine influenza using genesig Equine Flu H3N8&H7N7 kit, EHV-1, EHV-4 and Streptococcus equi using the respective genesig kits.
Lee SK, Choi J, Yoon J, Jung J, Park JY, Park J, Kim Y, Park JY, Park D. Molecular Detection of Equine Adenovirus 1 in Nasal Swabs from Horses in the Republic of Korea. Vet Sci. 2022 Apr 13;9(4):187. doi: 10.3390/vetsci9040187. PMID: 35448685; PMCID: PMC9025330.
Blood samples from horses and donkeys were tested for EIAV proviral DNA with the commercial real-time PCR kits (genesig EIAV-Easy Kit and genesig EIAV-Standard Kit).
Alnaeem AA, Hemida MG. Surveillance of the equine infectious anemia virus in Eastern and Central Saudi Arabia during 2014-2016. Vet World. 2019 May;12(5):719-723. doi: 10.14202/vetworld.2019.719-723. Epub 2019 May 29. PMID: 31327910; PMCID: PMC6584864.
Nasal swabs, lungs and spleens of dead horses that had displayed respiratory signs; and liver of aborted foetuses) were collected and tested for EHV-1 (genesig advanced kit), EHV-4 (genesig advanced kit), and Equine/Canine influenza [H3N8&H7N7] (genesig standard kit).
Ghoniem SM, El Deeb AH, Aggour MG, Hussein HA. Development and evaluation of a multiplex reverse-transcription real-time PCR assay for detection of equine respiratory disease viruses. J Vet Diagn Invest. 2018 Nov;30(6):924-928. doi: 10.1177/1040638718799388. Epub 2018 Sep 21. PMID: 30239276; PMCID: PMC6505849.
Samples from case-patients, unpasteurized milk and dairy products, tested using genesig real-time PCR kits for S. equi subsp. Zooepidemicus.
Bosica S, Chiaverini A, De Angelis ME, Petrini A, Averaimo D, Martino M, Rulli M, Saletti MA, Cantelmi MC, Ruggeri F, Lodi F, Calistri P, Cito F, Cammà C, Di Domenico M, Rinaldi A, Fazii P, Cedrone F, Di Martino G, Accorsi P, Morelli D, De Luca N, Pomilio F, Parruti G, Savini G. Severe Streptococcus equi Subspecies zooepidemicus Outbreak from Unpasteurized Dairy Product Consumption, Italy. Emerg Infect Dis. 2023 May;29(5):1020-1024. doi: 10.3201/eid2905.221338. PMID: 37081588; PMCID: PMC10124651.
Lungs, liver, spleen, retropharyngeal lymph nodes, and guttural pouch samples from donkeys were obtained aseptically and were used for routine aerobic and microaerophilic bacterial cultures. Cultures were tested using genesig real-time PCR kits for S. equi subsp. Zooepidemicus.
Cantelmi MC, Merola C, Averaimo D, Chiaverini A, Cito F, Cocco A, Di Teodoro G, De Angelis ME, Di Bernardo D, Auzino D, Petrini A. Identification of the Novel Streptococcus equi subsp. zooepidemicus Sequence Type 525 in Donkeys of Abruzzo Region, Italy. Pathogens. 2023 May 23;12(6):750. doi: 10.3390/pathogens12060750. PMID: 37375440; PMCID: PMC10305129.
(not testing equine samples but using Klebsiella Pneumonia kit, which is also relevant in equine industry)
Mouse fecal and cecal samples were tested and quantification of Kp was performed using a Klebsiella pneumonia-EASY Genesig Kit (Primerdesign Ltd., UK), PrecisionPLUS qPCR Master Mix (Primerdesign Ltd.), and the Thermal Cycler Dice Real-Time System II (Takara).
Ichikawa M, Nakamoto N, Kredo-Russo S, Weinstock E, Weiner IN, Khabra E, Ben-Ishai N, Inbar D, Kowalsman N, Mordoch R, Nicenboim J, Golembo M, Zak N, Jablonska J, Sberro-Livnat H, Navok S, Buchshtab N, Suzuki T, Miyamoto K, Teratani T, Fujimori S, Aoto Y, Konda M, Hayashi N, Chu PS, Taniki N, Morikawa R, Kasuga R, Tabuchi T, Sugimoto S, Mikami Y, Shiota A, Bassan M, Kanai T. Bacteriophage therapy against pathological Klebsiella pneumoniae ameliorates the course of primary sclerosing cholangitis. Nat Commun. 2023 Jun 5;14(1):3261. doi: 10.1038/s41467-023-39029-9. PMID: 37277351; PMCID: PMC10241881.
