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Our Internal Control kits can be used alongside our genesig kits or your own assay, either as a full workflow control to confirm the extraction efficiency and quality of extracted sample, or as an internal run control for the qPCR reaction itself.
The RNA template serves as an exogenous control that is spiked into the sample during the extraction process or into the PCR reaction mix.
The primer/probe mix included in the kit allows detection of the RNA template in the qPCR reaction, without interfering with the detection of the main target.
A number of different dyes are available so that a suitable channel can be used to detect the internal control alongside your main assay. Please select a dye that reads through a separate channel than your main target.
Please note, kits with a ‘LL’ suffix use a different internal control sequence and are mainly recommended for applications using samples derived from rat, for fast cycling conditions, or in workflows where the standard internal control assay may not be suitable.
At Primerdesign, we are proud to sell our products internationally. We work hard to keep shipping costs to a minimum. Kits are normally sent by courier to ensure rapid delivery. Cost varies according to your location. When ordering through our online shop, the delivery cost will be calculated based on your location and items in your basket (ambient or dry ice shipping conditions) and any additional shipping items you purchase.
You will have the option to purchase through Primerdesign directly, or through a trusted distributor. Click here to check if we have a local distributor in your territory.
Estimated delivery depends on whether the items in your basket have a shipping time of 5 working days or 4 weeks. Please note, this timeframe excludes the 24-48 hours for Customer Services to process your order.
If you are a new customer placing your first order with Primerdesign, please note there may be a short delay to your order processing time whilst we conduct necessary checks.
Please note, Certificate of Origin Requests and Additional Paperwork requests may cause delays in shipment processing times.