Toxoplasma gondii 529 (Maximum Sensitivity)
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Toxoplasma gondii is a species of parasitic protozoa in the genus Toxoplasma, and the causative agent of toxoplasmosis.
T.gondii_ 529-bp repeat element: For maximum sensitivity
This kit targets the 529-bp repeat element, which is reported to be present at 200-300 copies/genome, providing maximum sensitivity of detection. Due to the genomic variance of the target region, only 75% of T.gondii sequences available on the NCBI database (at time of last design review) are predicted to be detected.
Primerdesign Ltd also offers an additional Toxoplasma gondii detection kit that targets the B1 gene for maximum coverage.
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DNA amplification was carried out using Primerdesign™ Ltd., and T. gondii repeat region genesig® Standard kit and oasig™ lyophilized 2× qPCR Mastermix (Uk) qPCR were performed for DNA amplification in final volume 20 μl. The amplification was performed in Mx3000P platforms.
Saad NM, Hussein AAA, Ewida RM. Occurrence of Toxoplasma gondii in raw goat, sheep, and camel milk in Upper Egypt. Vet World. 2018 Sep;11(9):1262-1265. doi: 10.14202/vetworld.2018.1262-1265. Epub 2018 Sep 15. PMID: 30410231; PMCID: PMC6200564.
Occurrence of Toxoplasma gondii in raw goat, sheep, and camel milk in Upper Egypt - PMC (nih.gov)
Purified DNA was then tested for the presence of Chlamydia psittaci (Primerdesign Ltd Chlamydia psittaci, gidA gene—genesig Advanced Kit, Rownhams, UK), Listeria spp. (Primerdesign Ltd Lysteria, Invasion-associated Protein p60 (iap) gene—genesig Advanced Kit, Rownhams, UK), Neospora caninum (Primerdesign Ltd Neospora caninum, Nc5 marker genomic sequence—genesig Advanced Kit, Rownhams, UK), and Toxoplasma gondii (Primerdesign Ltd Toxoplasma gondii, Repeat region—genesig Advanced Kit, Rownhams, UK).
Zaccaria G, Lorusso A, Hierweger MM, Malatesta D, Defourny SV, Ruggeri F, Cammà C, Ricci P, Domenico MD, Rinaldi A, Decaro N, D'Alterio N, Petrini A, Seuberlich T, Marcacci M. Detection of Astrovirus in a Cow with Neurological Signs by Nanopore Technology, Italy. Viruses. 2020 May 11;12(5):530. doi: 10.3390/v12050530. PMID: 32403368; PMCID: PMC7290991.
Molecular detection of abortive agents (Brucella spp, T. gondii, N. caninum, Schmallenberg virus, Leptospira spp. Chlamydiacae, BDV and BTV) was carried out on the samples taken from the fetus and two lambs with neurological symptoms. DNA extraction was executed with a Maxwell® 16 Cell and Tissue DNA Purification Kit (Promega Italia Srl, Milan, Italy). The DNA purity was checked by NanoDrop2000 (ThermoFisher Scientific, Waltham, MA, USA). The mix was prepared using the Genesig Advanced Kit (Genesig, York House, School Lane, Chandler’s Ford, UK), according to the manufacturer’s instructions, and Real-Time PCR was performed (QuantStudio™ 7 Pro Applied Biosystems, CA, USA).
De Angelis ME, Martino C, Chiaverini A, Di Pancrazio C, Di Marzio V, Bosica S, Malatesta D, Salucci S, Sulli N, Acciari VA, Pomilio F. Co-Infection of L. monocytogenes and Toxoplasma gondii in a Sheep Flock Causing Abortion and Lamb Deaths. Microorganisms. 2022 Aug 15;10(8):1647. doi: 10.3390/microorganisms10081647. PMID: 36014064; PMCID: PMC9415574.
quantitative PCR was performed on CSF samples for the detection of Neospora caninum and Toxoplasma gondii using N caninum Nc5 marker genomic sequence assays and Genesig T gondii repeat region, respectively. Assays were performed by Langford Vets Diagnostic Lab on a Qtower3 (Analytik Jena, Germany) following a laboratory‐validated thermocycling protocol.
Jones BS, Harcourt-Brown T. Comparison of serum creatine kinase and aspartate aminotransferase activity in dogs with Neospora meningoencephalitis and noninfectious meningoencephalitis. J Vet Intern Med. 2022 Jan;36(1):141-145. doi: 10.1111/jvim.16334. Epub 2021 Dec 3. PMID: 34859908; PMCID: PMC8783338.
