genesig Easy DNA/RNA Extraction Kit
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The genesig easy DNA/RNA extraction protocol begins with a simple lysis step where cells and tissue are lysed to release their nucleic acid. Then minute magnetic particles are added to bind to DNA/RNA. When placed on to the genesig magnetic separator the particles are pulled to the side of the tube making it easy to remove the unwanted supernatant with a pipette. Then a series of simple wash steps are performed before the DNA/RNA is washed off the beads back in to solution, ready for analysis by real-time PCR.
Its fast, and incredibly easy to perform.
Suitable sample types:
- Clinical and Veterinary - including whole blood, plasma, and serum; saliva and sputum; faeces and urine; tissues; dry swabs; and bacterial culture broth
- Food - including meat, fish, milk, and cooked or processed food products
- Environmental - including plant, soil, and water
At Primerdesign, we are proud to sell our products internationally. We work hard to keep shipping costs to a minimum. Kits are normally sent by courier to ensure rapid delivery. Cost varies according to your location. When ordering through our online shop, the delivery cost will be calculated based on your location and items in your basket (ambient or dry ice shipping conditions) and any additional shipping items you purchase.
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Please note, Certificate of Origin Requests and Additional Paperwork requests may cause delays in shipment processing times.
Extraction from tissue samples from the spleen, liver and brain
Alathari S, Chaput DL, Bolaños LM, Joseph A, Jackson VLN, Verner-Jeffreys D, Paley R, Tyler CR, Temperton B. A Multiplexed, Tiled PCR Method for Rapid Whole-Genome Sequencing of Infectious Spleen and Kidney Necrosis Virus (ISKNV) in Tilapia. Viruses. 2023 Apr 14;15(4):965. doi: 10.3390/v15040965. Erratum in: Viruses. 2023 Jun 29;15(7): PMID: 37112945; PMCID: PMC10145788.
Extraction from whole blood (EDTA)
Gummery L, Jallow S, Raftery AG, Bennet E, Rodgers J, Sutton DGM. Comparison of loop-mediated isothermal amplification (LAMP) and PCR for the diagnosis of infection with Trypanosoma brucei ssp. in equids in The Gambia. PLoS One. 2020 Aug 24;15(8):e0237187. doi: 10.1371/journal.pone.0237187. PMID: 32833981; PMCID: PMC7444819.
Extracting from cell culture supernatant – Also using genesig C.trachomatis Advanced kit and PrecisionPLUS mastermix
Yang S, Traore Y, Jimenez C, Ho EA. Autophagy induction and PDGFR-β knockdown by siRNA-encapsulated nanoparticles reduce chlamydia trachomatis infection. Sci Rep. 2019 Feb 4;9(1):1306. doi: 10.1038/s41598-018-36601-y. PMID: 30718536; PMCID: PMC6361950.
Extracting RNA from fecal samples of sheep and goats
AlAzemi MS, Majeed QAH, Samy A, Henedi AA, Youssef W, Abdou NMI. Evaluation of immunochromatography test for detection of four enteropathogens in the feces of sheep and goats in Kuwait. Open Vet J. 2021 Jul-Sep;11(3):500-507. doi: 10.5455/OVJ.2021.v11.i3.23. Epub 2021 Sep 17. PMID: 34722215; PMCID: PMC8541720.
Extraction from cultures, and fecal samples from pigs
Wu Y, Tian K, Zhang Y, Guo H, Li N, Wang Z, Zhao J. Rapid and visual detection of Lawsonia intracellularis with an improved recombinase polymerase amplification assay combined with a lateral flow dipstick. BMC Vet Res. 2019 Mar 21;15(1):97. doi: 10.1186/s12917-019-1841-9. PMID: 30898117; PMCID: PMC6429820.
Extraction from bacterial cultures
Del Rio-Rodriguez RE, Ramirez-Paredes JG, Soto-Rodriguez SA, Shapira Y, Huchin-Cortes MDJ, Ruiz-Hernandez J, Gomez-Solano MI, Haydon DJ. First evidence of fish nocardiosis in Mexico caused by Nocardia seriolae in farmed red drum (Sciaenops ocellatus, Linnaeus). J Fish Dis. 2021 Aug;44(8):1117-1130. doi: 10.1111/jfd.13373. Epub 2021 Apr 13. PMID: 33848372.
Extraction from pure cultures of P. acnes grown in BHI or THIO media
Neama Ahmad Alnabati, Ahmed Mahmoud Al-Hejin, Samah Omar Noor, Mohamed Morsi M. Ahmed, Mohamed Abu-Zeid & Nouf Talal Mleeh (2021) The antibacterial activity of four Saudi medicinal plants against clinical isolates of Propionibacterium acnes, Biotechnology & Biotechnological Equipment, 35:1, 415-424, DOI: 10.1080/13102818.2021.1885992