PrecisionMULTIPLEX OneStep RT-qPCR Master Mix

Optimised formulation for enhanced multiplex reactions



  • At least 50% increase in throughput
  • Precise reproducible results when multiplexing up to 4 targets in single well
  • Suitable for both RNA and DNA targets
  • Optimised to run on fast and standard cycling protocols
  • Exceptional value for money
Available for direct shipping to United Kingdom
Est. dispatch: < 2 days

PrecisionMULTIPLEX OneStep RT-qPCR Master Mix can help when you have many different tests to run, or if you have a limited number of machines. This 2X Master Mix contains a unique formulation to maximise the processing capacity, enabling detection of up to 4 RNA or DNA targets simultaneously.

The core components are a hotstart Taq polymerase and a Reverse Transcriptase enzyme, including an ammonium sulphate based buffer and a special additive to reduce primer-dimers. Our unique formulation gives a superior quality of data with a bigger signal to noise ratio, brighter, steeper and more precise amplification plots. Stabilisers and preservatives ensure that multiple freeze-thaw cycles do not affect the performance giving you reliable, reproducible results.

PrecisionMULTIPLEX OneStep RT-qPCR Master Mix is available for all machines - see the technical details tab for a list of qPCR machines and their individual specifications.

Primerdesign Real-Time PCR reagents are manufactured to the highest standards within our ISO9001:2008 and ISO13485:2012 certified quality management laboratory environment.

PrecisionMULTIPLEX OneStep RT-qPCR Master Mix contains a unique ultra-fast processing enzyme that can be run on fast or standard cycling protocols. If using fast cycling this will allow run times to be drastically reduced - 40 minutes is typical compared to 1-2 hours with standard cycling protocols. 

To ensure maximum quality PrecisionMULTIPLEX OneStep RT-qPCR Master Mix is offered in slightly altered formulations optimised for different Real-Time PCR machines.

Real-time PCR machine

Description

Cat no.

ABI 7000

PrecisionMULTIPLEX OneStep RT-qPCR with ROX

OSMULTIPLEX-R-10ML

ABI 7300

PrecisionMULTIPLEX OneStep RT-qPCR with ROX

OSMULTIPLEX-R-10ML

ABI 7500

PrecisionMULTIPLEX OneStep RT-qPCR with low ROX

OSMULTIPLEX-LR-10ML

ABI 7700

PrecisionMULTIPLEX OneStep RT-qPCR with ROX

OSMULTIPLEX-R-10ML

ABI 7900

PrecisionMULTIPLEX OneStep RT-qPCR with ROX

OSMULTIPLEX-R-10ML

ABI ViiA7

PrecisionMULTIPLEX OneStep RT-qPCR with low ROX

OSMULTIPLEX-LR-10ML

ABI StepOne

PrecisionMULTIPLEX OneStep RT-qPCR with ROX

OSMULTIPLEX-R-10ML

ABI StepOnePLUS

PrecisionMULTIPLEX OneStep RT-qPCR with ROX

OSMULTIPLEX-R-10ML

ABI GeneAmp 5700

PrecisionMULTIPLEX OneStep RT-qPCR with ROX

OSMULTIPLEX-R-10ML

Life Technologies QuantStudio

PrecisionMULTIPLEX OneStep RT-qPCR with low ROX

OSMULTIPLEX-LR-10ML

BioRad iCycler (all models)

PrecisionMULTIPLEX OneStep RT-qPCR for BioRad

OSMULTIPLEX-iC-10ML

BioRad CFX96

PrecisionMULTIPLEX OneStep RT-qPCR for BioRad

OSMULTIPLEX-10ML

Stratagene MX (all models)

PrecisionMULTIPLEX OneStep RT-qPCR for Stratagene

OSMULTIPLEX-MX-10ML

   Opticon and Chromo4 (all models)

PrecisionMULTIPLEX OneStep RT-qPCR MasterMix

OSMULTIPLEX-10ML

RotorGene (all models)

PrecisionMULTIPLEX OneStep RT-qPCR MasterMix

OSMULTIPLEX-10ML

Roche LightCycler 480

PrecisionMULTIPLEX OneStep RT-qPCR MasterMix

OSMULTIPLEX-10ML

Roche LightCycler 1.0-2.0

PrecisionMULTIPLEX OneStep RT-qPCR for LightCyclers

  OSMULTIPLEX-CL-10ML

Roche LightCycler LC96

PrecisionMULTIPLEX OneStep RT-qPCR MasterMix

OSMULTIPLEX-10ML

Eppendorf Mastercycler

PrecisionMULTIPLEX OneStep RT-qPCR MasterMix

OSMULTIPLEX-10ML

Fluidigm BioMark

PrecisionMULTIPLEX OneStep RT-qPCR MasterMix

OSMULTIPLEX-10ML

Cepheid SmartCycler

PrecisionMULTIPLEX OneStep RT-qPCR MasterMix

OSMULTIPLEX-10ML

Illumina Eco

PrecisionMULTIPLEX OneStep RT-qPCR MasterMix

OSMULTIPLEX-10ML

PCRMax Eco

PrecisionMULTIPLEX OneStep RT-qPCR MasterMix

OSMULTIPLEX-10ML

Thermo PikoReal

PrecisionMULTIPLEX OneStep RT-qPCR MasterMix

OSMULTIPLEX-10ML

PrimePro 48

PrecisionMULTIPLEX OneStep RT-qPCR MasterMix

OSMULTIPLEX-10ML

Analytik Jena qTower Series

PrecisionMULTIPLEX OneStep RT-qPCR MasterMix

OSMULTIPLEX-10ML

Any other

Please ask

Please ask

 

 

Q: Are samples available?

A:  Yes.  Let us know which machine you will be working via our “Ask a Question” form, and we will arrange for a free sample to be sent out immediately.  Please do let us know how your testing goes.  

Q: Will this work on my machine?

A: Yes.  We can support any real-time PCR machine.  We sell slightly different formulations for different machines.  Simply select your machine during online purchase, or let us know which machine you are using via our “Ask a Question” form and we will ensure that we quote you for the correct product/catalogue number.


Purchase options

PrecisionMULTIPLEX OneStep RT-qPCR Master Mix

Catalogue no: OSMULTIPLEX-machine type-10ML

10 x 1ml aliquots of PrecisionMULTIPLEX OneStep RT-qPCR Master Mix.

£1,111.37
Kit For probe based detection
Species
Number of biobank samples
Additional requirements
Gene symbol
Select your machine
cDNA tissue source
cDNA cell line source
cDNA primary cell source
Probe fluorophore channel
Probe type
150 reaction kit
400 reaction kit
MYPLEX
Quantity

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