PrecisionPLUS OneStep RT-qPCR Master Mix

High quality, competitively priced



  • Huge cost saving
  • As good or better than leading brands
  • Precise reproducible results
  • Available premixed with SYBR green at no extra charge
Available for direct shipping to United Kingdom
Est. dispatch: < 2 days

Our new PrecisionPLUS OneStep RT-qPCR Master Mix is available now. This new kit contains a higher fidelity Taq enzyme which further reduces the possibility of primer dimer formation. In addition, we have switched from a chemical hotstart Taq to an antibody modification in PrecisionPLUS OneStep and thus have been able to reduce the enzyme activation time resulting in shorter run times. As a consequence, you will see the same or better data, in a shorter time.

Our PrecisionPLUS OneStep RT-qPCR Master Mix is a convenient, one step, closed tube reaction which means reduced setup/handling time as well as reduced risk of contamination. Our optimised rapid protocol includes a 10 minute RT step. It is also ideal for RNA pathogen detection. Just add primers and template for a perfect real-time PCR reaction. 

Primerdesign real-time PCR reagents are manufactured to the highest standards within our ISO9001:2008 and ISO13485:2012 certified quality management laboratory environment.

This products contains an especially modified reverse transcriptase enzyme as well as the Taq polmerase thus only requiring the addition of RNA to the mix. Supplied with an enzyme free mix which allows an RT negative control giving you complete scientific control and integrity. 

To use this you simply add your RNA sample to the mix and include an additional incubation at 55C for 10 minutes prior to your qPCR program cycle.

Real-time PCR machine

Description

Cat no.

ABI 7000

PrecisionPLUS OneStep RT-qPCR with ROX

OSPLUS-R-XXML*

ABI 7300

PrecisionPLUS OneStep RT-qPCR with ROX

OSPLUS-R-XXML*

ABI 7500

PrecisionPLUS OneStep RT-qPCR with low ROX

OSPLUS-LR-XXML*

ABI 7700

PrecisionPLUS OneStep RT-qPCR with ROX

OSPLUS-R-XXML*

ABI 7900

PrecisionPLUS OneStep RT-qPCR with ROX

OSPLUS-R-XXML*

                         ABI ViiA7

PrecisionPLUS OneStep RT-qPCR with low ROX

OSPLUS-LR-XXML*

ABI StepOne

PrecisionPLUS OneStep RT-qPCR with ROX

OSPLUS-R-XXML*

ABI StepOnePLUS

PrecisionPLUS OneStep RT-qPCR with ROX

OSPLUS-R-XXML*

ABI GeneAmp 5700

PrecisionPLUS OneStep RT-qPCR with ROX

OSPLUS-R-XXML*

Life Technologies QuantStudio

PrecisionPLUS OneStep RT-qPCR with low ROX

OSPLUS-LR-XXML*

BioRad iCycler (all models)

PrecisionPLUS OneStep RT-qPCR for BioRad

OSPLUS-iC-XXML*

BioRad CFX96

PrecisionPLUS OneStep RT-qPCR Master Mix

OSPLUS-XXML*

Stratagene MX (all models)

PrecisionPLUS OneStep RT-qPCR for Stratagene

OSPLUS-MX-XXML*

   Opticon and Chromo4 (all models)

PrecisionPLUS OneStep RT-qPCR Master Mix

OSPLUS-XXML*

RotorGene (all models)

PrecisionPLUS OneStep RT-qPCR Master Mix

OSPLUS-XXML*

Roche LightCycler 480

PrecisionPLUS OneStep RT-qPCR Master Mix

OSPLUS-XXML*

Roche LightCycler 1.0-2.0

PrecisionPLUS OneStep RT-qPCR for LightCyclers

OSPLUS-CL-XXML*

Roche LightCycler LC96

PrecisionPLUS OneStep RT-qPCR Master Mix

OSPLUS-XXML*

Eppendorf Mastercycler

PrecisionPLUS OneStep RT-qPCR Master Mix

OSPLUS-XXML*

Fluidigm BioMark

PrecisionPLUS OneStep RT-qPCR Master Mix

OSPLUS-XXML*

Cepheid SmartCycler

PrecisionPLUS OneStep RT-qPCR Master Mix

OSPLUS-XXML*

 Illumina Eco

PrecisionPLUS OneStep RT-qPCR Master Mix

OSPLUS-XXML*

PCRMax Eco

PrecisionPLUS OneStep RT-qPCR Master Mix

OSPLUS-XXML*

Thermo PikoReal

PrecisionPLUS OneStep RT-qPCR Master Mix

OSPLUS-XXML*

Any other

Please ask

Please ask

*Please replace the XX with the volume required: 1ML, 2ML, 5ML, 10ML, or 20ML.


Purchase options

PrecisionPLUS OneStep RT-qPCR Master Mix

Catalogue no: OSPLUS-machine type-1ML

1 x 1ml aliquot of PrecisionPLUS OneStep RT-qPCR Master Mix.

£115.36
Kit For probe based detection
Species

Premixed with SYBR green

Number of biobank samples
Additional requirements
Gene symbol
Select your machine
cDNA tissue source
cDNA cell line source
cDNA primary cell source
Probe fluorophore channel
Probe type
150 reaction kit
400 reaction kit
MYPLEX
Quantity

Add to basket

PrecisionPLUS OneStep RT-qPCR Master Mix

Catalogue no: OSPLUS-machine type-2ML

2 x 1ml aliquots of PrecisionPLUS OneStep RT-qPCR Master Mix.

£204.97
Kit For probe based detection
Species

Premixed with SYBR green

Number of biobank samples
Additional requirements
Gene symbol
Select your machine
cDNA tissue source
cDNA cell line source
cDNA primary cell source
Probe fluorophore channel
Probe type
150 reaction kit
400 reaction kit
MYPLEX
Quantity

Add to basket

PrecisionPLUS OneStep RT-qPCR Master Mix

Catalogue no: OSPLUS-machine type-5ML

5 x 1ml aliquots of PrecisionPLUS OneStep RT-qPCR Master Mix.

£501.61
Kit For probe based detection
Species

Premixed with SYBR green

Number of biobank samples
Additional requirements
Gene symbol
Select your machine
cDNA tissue source
cDNA cell line source
cDNA primary cell source
Probe fluorophore channel
Probe type
150 reaction kit
400 reaction kit
MYPLEX
Quantity

Add to basket

PrecisionPLUS OneStep RT-qPCR Master Mix

Catalogue no: OSPLUS-machine type-10ML

10 x 1ml aliquots of PrecisionPLUS OneStep RT-qPCR Master Mix.

£763.23
Kit For probe based detection
Species

Premixed with SYBR green

Number of biobank samples
Additional requirements
Gene symbol
Select your machine
cDNA tissue source
cDNA cell line source
cDNA primary cell source
Probe fluorophore channel
Probe type
150 reaction kit
400 reaction kit
MYPLEX
Quantity

Add to basket

PrecisionPLUS OneStep RT-qPCR Master Mix

Catalogue no: OSPLUS-machine type-20ML

20 x 1ml aliquots of PrecisionPLUS OneStep RT-qPCR Master Mix.

£1,442
Kit For probe based detection
Species

Premixed with SYBR green

Number of biobank samples
Additional requirements
Gene symbol
Select your machine
cDNA tissue source
cDNA cell line source
cDNA primary cell source
Probe fluorophore channel
Probe type
150 reaction kit
400 reaction kit
MYPLEX
Quantity

Add to basket

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