oasig Lyophilised Master Mixes

Bacterial DNA was quantified by application of Eubacteria genesig® Standard Kit and the oasig lyophilised 2x qPCR Master Mix (Primerdesign Ltd.).

Bick A, Buys W, Engler A, Madel R, Atia M, Faro F, Westendorf AM, Limmer A, Buer J, Herbstreit F, Kirschning CJ, Peters J. Immune hyporeactivity to bacteria and multiple TLR-ligands, yet no response to checkpoint inhibition in patients just after meeting Sepsis-3 criteria. PLoS One. 2022 Aug 18;17(8):e0273247. doi: 10.1371/journal.pone.0273247. PMID: 35981050; PMCID: PMC9387870.

Immune hyporeactivity to bacteria and multiple TLR-ligands, yet no response to checkpoint inhibition in patients just after meeting Sepsis-3 criteria - PMC (nih.gov)

The qPCR assay was performed using C. difficile Genesig Advanced Kit (PrimerDesign, Camberley, UK). The amplification reactions were carried out in a final volume of 20 μL, which consisted of 5 μL of the template and 15 μL of the master mix, containing Oasig 2X qPCR Master Mix, 300 nM of each primer and 150 nM fluorescence-labeled probe and distilled water. The assay was performed with the CFX Touch Real-Time PCR Detection System.

Sleha R, Radochova V, Mikyska A, Houska M, Bolehovska R, Janovska S, Pejchal J, Muckova L, Cermak P, Bostik P. Strong Antimicrobial Effects of Xanthohumol and Beta-Acids from Hops against Clostridioides difficile Infection In Vivo. Antibiotics (Basel). 2021 Apr 6;10(4):392. doi: 10.3390/antibiotics10040392. PMID: 33917416; PMCID: PMC8067520.

Strong Antimicrobial Effects of Xanthohumol and Beta-Acids from Hops against Clostridioides difficile Infection In Vivo - PMC (nih.gov)

Quantitative real-time PCR (qPCR) was performed using a 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster city, CA, USA) and a Hepatitis B Virus PCR Kit (MyBiosource, Inc., San Diego, CA, USA) with oasig lyophilized 2× qPCR Mastermix (Primerdesign, Ltd., Camberley, UK).

Um I, Choi S, Kim Y, Pang K, Lee J, Lee M, Kim B. Measurement of hepatitis B virus DNA in fresh versus processed dentin from chronically infected patients. J Transl Med. 2018 Dec 12;16(1):351. doi: 10.1186/s12967-018-1719-9. PMID: 30541555; PMCID: PMC6292124.

Measurement of hepatitis B virus DNA in fresh versus processed dentin from chronically infected patients - PMC (nih.gov)