Reverse Transcription Kits
The ultimate set of tools for cDNA synthesis
High efficiency Reverse Transcription (RT) ensures
the ability to detect rare transcripts and obtain the strongest possible real-time PCR signals. A complete solution, the PrimerDesign Reverse Transcription kits contain all of the reagents necessary for a perfect RT step.
Unique product features
- Cost saving
- Precise, reproducible results
- Operates over a wide range of temperatures up to 55C
- Simple, two step RT protocol
- Flexible kit design- Oligo-dT, random nonamer or gene specific RT priming
Currently available in Europe only
Versatile, top spec Reverse Transcription Kits supplied as separate components. Our nanoScript and picoScript RT kits allow for maximum versatility. Ideal for those who need to chose an ideal priming strategy (oligo dT, random, gene specific) or for challenging sources of RNA.
Our Reverse Transcriptase enzyme is especially modified to work at high temperatures. Higher reaction temperatures reduce the RNA secondary structure (tangling) and allow the production of longer transcripts and make for faster reactions. The enzyme also has an increased affinity for primer-template complexes which allow for efficient transcription of very low amounts of RNA.
Although PrimerDesign recommends Oligo-dT priming for most applications, a random nonamer primer is included in the kit in case this is your preferred strategy.
Has the name of this product changed? Yes. To avoid confusion with a similarly named product on the market the names of our RT kits have changed to become 'nanoScript' and 'picoScript' as of June 2011. The product remains the same high quality solution for cDNA synthesis, but simply with a new label on.
Kit contents:
High quality nanoScript/picoScript RT enzyme, 10 x RT buffer, DTT, oligo-dT primers, random nonamer primers, dNTPs, RNAse/DNAse-free water.