Specialist products positive control - Real Time PCR positive control

Real-time PCR internal control kit

Optimal positive control for DNA/RNA extraction and PCR conditions

Negative test results are only valid when the suitability of the reagents, template and protocols have also been demonstrated. The PrimerDesign real-time PCR internal control kit contains either control RNA or DNA. This can be spiked into your sample prior to nucleic acid extraction or diluted and spiked directly in to your real-time PCR reaction. A positive signal of around Ct 25 indicates that your experimental procedure is working well.

The internal control kit can be multiplexed, or used in parallel with your test samples. The assay is primer limited and based on an obscure nucleic acid sequence so will not therefore interfere with the detection of you target sequence.

Product features

Internal PCR control
Taqman style probe
Available with CY5, FAM, TAMRA or Yakima Yellow probe.
Primer limited to prevent interference with target gene primers

Kit contents
Lyophilised positive control primers/probe, positive control DNA or RNA template, PCR grade water.

Sample data (click for details)

HCV with internal extraction control

Int-DNA-YY	Internal PCR DNA control with Yakima Yellow probe	click
Int-DNA-CY5	Internal PCR DNA control with CY5 probe	click
Int-DNA-FAM	Internal PCR DNA control with FAM probe	click
Int-DNA-TAMRA	Internal PCR DNA control with TAMRA probe	click

Int-RNA-YY	Internal PCR RNA control with Yakima Yellow probe	click
Int-RNA-CY5	Internal PCR RNA control with CY5 probe	click
Int-RNA-FAM	Internal PCR RNA control with FAM probe	click
Int-RNA-TAMRA	Internal PCR RNA control with TAMRA probe	click

ORDER ONLINE, E:ORDERS@PRIMERDESIGN.CO.UK, T: +44 (0)2380 312777, F: +44 (0)8708 362155