| geNorm reference gene selection kits | ||
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Rapidly improve the quality of your real-time PCR data |
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More than 2000 papers have cited the geNorm method. For accurate gene quantification, it is essential to normalise real-time PCR data to a fixed reference; one that is not affected by your experimental conditions. There is increasing evidence that normalising to a single, randomly selected reference (house-keeping) gene e.g. Actin Beta, introduces large and variable errors into the analysis. geNorm is a system for selecting the best candidate reference gene for a given experimental scenario. To perform geNorm analysis the user measures the expression of 6 or 12 reference (house-keeping) genes in a representative set of their own samples. The geNorm software provided with the kit ranks the reference (house-keeping) genes in order of stability of expression. The output graphs and report simply identify the bestreference (house-keeping) genes for that particular experiment. Full details of how this kit works and why it is so important can be found in this technical bulletin. |
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Each kit inlcudes 3 months free access (download instructions and activation code) to the geNorm analysis tool in qbasePLUS, the world's leading real-time PCR data analysis software from Biogazelle. |
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