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| Overview of our geNorm services |
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geNorm kit selects the best normalising genes for your experiment and improves the quality of your real time PCR data.
More than 500 papers have cited the geNorm method.
For accurate gene quantitation, it is essential to normalise real-time PCR data to a fixed reference; one that is not affected by your experimental conditions. There is increasing evidence that normalising to a single, randomly selected housekeeping gene e.g. Actin Beta, introduces large and variable errors into the analysis. geNorm is a system for selecting the best candidate reference gene for each individual experimental scenario.
The geNorm kit
Simply measure the expression of the 6 or 12 candidate housekeeping genes in the kit in a number of your samples. Plug the data in to the geNorm software provided and geNorm will calculate the most stably expressed housekeeping genes in your experimental model. The net result of using the correct normalising controls is an increase in the accuracy, sensitivity and reproducibility of your data.
geNorm kits are available for a large range of species. Click for more information.
Technical bulletin with example geNorm data
Any questions? Please contact us
Life after geNorm...
Having used geNorm to select the best normalising genes for your experiment, PrimerDesign can provide ready to use high quality housekeeping gene detection kits for your next experiments.
genorm customers qualify for a 50% discount-just ask.
geNorm licences
geNorm is free for academic users. Commercial entities require a licence. Please click for details.
References
Centre for Medical Genetics at Gent University Hospital - More geNorm information
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Vandesompele et al 2002. -Original geNorm article. [n° 4 in ranking of all time most-viewed articles published by BioMed Central]
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ORDER ONLINE, E:ORDERS@PRIMERDESIGN.CO.UK, T: +44 (0)2380 473274, F: +44 (0)8708 362 155
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