One of the greatest benefits you will find in using our services is the flexibly we can offer at the level of assay design. We appreciate that many of our customers no long wish to just measure global expression levels, but to elucidate with splice forms are abundant and biologically relevant.
We are are well equipped with our expert team to assist these types of projects. Often specific design requests are included in the standard cost of the assay. Here is a list of requests that you can make that are free of charge:
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Design my assay close to the 3'end.
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Design my assay to detect this specific exon.
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Design my assay to span at least one intron.
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Design a single exon assay so that I can detect both cDNA and genomic DNA.
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Design my assay to detect gene x and make sure it wont detect gene y which is closely related.
Splice variants
Designing assays for specific splice variant detection has no additional cost. However, splice variant detection assays are supplied with a positive control which caries a small extra charge. This is for your benefit. Many splice variants that are sited in papers or submitted to the main sequence databases are expressed at very low levels or not at all in most tissues and circumstances. Our positive control enables you to prepare a copy number standard curve and also to quickly eliminate from your study splice variants that are not relevant. From our point of view, it is very hard for us to find the correct cDNA from our BioBank to validate splice variant specific assays. A synthetic positive control template enables us to properly validate your assay whilst also providing you the positive control you will need should to get conclusive results fast.
Predicted genes
If you are ordering a custom real-time PCR assay for a PREDICTED gene from the NCBI database we will also supply a synthetic copy number standard curve at a small cost. This is required because we cannot be sure of the authenticity of predicted sequences and thus we require a guaranteed template to validate the performance of the primers on.
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