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Products for real-time PCR
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Products for real-time PCR
pathogen detection

  Precision Reverse Transcription kits
 
New and improved high quality reverse transcription kits
 

High efficiency Reverse Transcription (RT) ensures the ability to detect rare transcripts and obtain the strongest possible real-time PCR signals. A complete solution, the PrimerDesign Reverse Transcription kits contain all the reagents necessary for the RT step. Although PrimerDesign recommends Oligo-dT priming for most applications, a random nonamer primer is included in the kit in case this is your preferred strategy.

 
rt
 

Our new Reverse Transcriptase enzyme is especially modified to work at high temperatures. Higher reaction temperatures reduce the RNA secondary structure (tangling) and allow the production of longer transcripts and make for faster reactions. The enzyme also has an increased affinity for primer-template complexes which allow for efficient transcription of very low amounts of RNA.

Looking for the old Product? If you are looking for our original Precision RT kit this has been replaced by the Precision qScript kit below at an identical price. However, the old product is available until the end of October 2009 for those who require it. Click here.



 

rel-time PCR kit

Unique product features

  • Cost saving
  • Precise, reproducible results
  • Operates over a wide range of temperatures up to 55C
  • Simple, two step RT protocol
  • Flexible kit design- Oligo-dT, random nonamer or gene specific RT priming

Kit contents
High quality qScript/picoScript RT enzyme, 10 x RT buffer, DTT, oligo-dT primers, random nonamer primers, dNTPs, RNAse/DNAse-free water.

Currently available in Europe only

 

 

  Precision qScript Reverse Transcription kits
   

Catalogue no: RT-qScript

50 reaction qScript Reverse Transcription kit suitable for 2 nanograms - 2 micrograms of RNA template


Quantity: 
Handbook download: PDF qPCR

   
  Precision picoScript Reverse Transcription kits
   

Catalogue no: RT-picoScript

50 reaction picoScript Reverse Transcription kit suitable for 3 picograms - 2 nanograms of RNA template


Quantity: 
Handbook download: PDF qPCR