The Precision OneStep™ qRT-PCR MasterMix contains all of the required components for a perfect one step real-time PCR analysis in a single reaction mix. The operator simply adds RNA and a primer/probe mix for a complete, closed tube, one step RNA to Ct reaction. nanoScript is a modified thermostable MMLV enzyme with an optimum working temperature of 55°C and a higher affinity for primer duplex initiation sites. This increases the rate of processing and the sensitivity of detection. The result is an RT step that is completed in less than 10 minutes and is sensitive in a linear range across the whole biological scope from very high (>1X109 copies) to very low (<10 copies) RNA copy numbers. An RNAse inhibitor is included to protect the sample from possible degradation. The amplification step components are based on our high quality Precision MasterMix product.

Download product bulletin